ANALGESIC, ANTI-INFLAMMATORY AND TOXICITY STUDIES ON METHANOLIC LEAF EXTRACT OF ROTHMANNIA LONGIFLORA SALISB (RUBIACEAE) IN RATS AND MICE
- Project Research
- 1-5 Chapters
- Abstract : Available
- Table of Content: Available
- Reference Style: APA
- Recommended for : Student Researchers
- NGN 3000
ABSTRACT
The herbalists in several African Countries have used Rothmannia longiflora decoctions as febrifugal and analgesic agent. The present study examined the analgesic, anti-inflammatory and toxicological profile of the methanolic extract of the plant in experimental animals. The preliminary phytochemical screening of the methanolic leaf extract of R.longiflora was carried out and revealed the presence of cardiac glycoside, steroids/terpenoids, tannins, saponins, flavonoids and carbohydrates. The acute toxic effect (median lethal dose values (LD50) of methanolic leaf extract of Rothmannia longiflora was carried out using Lork’s method in intraperitoneal and oral routes in mice and rats and were found to be above 5000mg/kg body weight. The anti-nociceptive effects of the extract was studied using acetic acid induced writhing test in mice, hot plate test in mice and formalin induced pain in rats. The anti-inflammatory effect was studied using Carrageenan induced paw oedema in rats. The extract at doses of 50-1000mg/kg significantly (P0.05) of the levels of Sodium (Na+ ), Potassium (K+ ), Chloride (Cl- ) ions, Urea and Creatinine compared to that of the control at the end of 30 days (sub-chronic) and 90 days (chronic) studies. In liver function test, there was no statistically significant difference (P>0.05) in the levels of AST, ALT, and ALP enzymes in all the treated groups compared to the control group after the 30 days studies. However, there was statistically significant difference with 500mg/kg and 1000mg/kg after the 90 days treatment. The Histopathological study showed no observable gross lesions during the periods of subchronic and chronic studies. vii These findings suggest that methanolic leaf extract of R.longiflora possesses analgesic and anti-inflammatory potentials/effects that justify the ethnomedical use of the plant in the treatment of painful and inflammatory conditions by the herbal practitioners. In addition, the results of toxicity studies showed that the extract is non-toxic to the kidneys and the heart during the sub-chronic and chronic toxicity studies however; mild fatty change was observed with 500mg/kg in the liver after the chronic studies.
Related Project Materials
EFFECTS OF FINANCIAL ACCOUNTING REPORTING ON MANAGERIAL DECISION MAKING
Abstract
Accounting reporting is necessary to understand financial situation of the firm and used as the basis of decis...
EFFECT OF GREEN TEA (CAMELLIA SINENSIS) ON THE PHARMACOKINETIC PROFILE OF CEPHALEXIN IN HEALTHY HUMAN VOLUNTEERS
ABSTRACT
Tea is a herb commonly consumed globally. Herbs are often taken concurrently with conventional medicines which results in increa...
EFFECTS OF AEROBIC TRAINING ON KIDNEY FUNCTION AMONG TYPE II DIABETES MELLITUS PATIENTS IN KANO METROPOLIS, NIGERIA
Abstract
Type 2 Diabetes is one of the most common physiological disorders worldwide. Kidney's role in and associated problems linked...
AN APPRAISAL OF THE LEGAL FRAMEWORK FOR INTERNATIONAL ENVIRONMENTAL PROTECTION UNDER THE LAW OF ARMED CONFLICT
Abstract
The effect of war has far gone beyond human suffering. Armed conflict has devastating effect on the environment. It is on record...
THE IMPACT MOTIVATION HAS ON EMPLOYEE PRODUCTIVITY
BACKGROUND TO THE STUDY
Although there is widespread consensus among psychologists that humans have a diverse set...
EFFECTIVE ADMINISTRATION OF SECONDARY SCHOOL AND ITS IMPPACT ON STUDENT ACADEMIC PERFORMANCE
BACKGROUND OF THE STUDY
Education remains the biggest instrument for academic progress, social mobiliza...
THE EFFECTS OF EMPLOYEE BENEFITS ON THE PERFORMANCE OF EMPLOYEES
ABSTRACT
Maintaining healthy employee relations in an organization is a pre-requisite for organizational success. One wa...
THE IMPLICATION OF DAVID HUME’S PHILOSOPHY OF IMPRESSIONS AND IDEAS
Abstract
Understanding the distinction between impressions and ideas that Hume draws in the opening paragraph of his &q...
IMPACT OF NIGERIAN TAX POLICIES ON THE ECONOMY AND SMALL BUSINESSES IN NIGERIA
Abstract- Small and Medium Enterprises play a very important role in development of the Nigerian Economy. Making up about 97% of t...
DESIGN AND IMPLEMENTATION OF E-LEARNING WEB PORTAL(A Case Study of the Department of Computer Science)
ABSTRACT
The tremendous impact of electronic technology (like computer and computer embedded devices, c...
